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Splicing together different regions of a gene by modified polymerase chain reaction-based site-directed mutagenesis
- Source :
-
Analytical Biochemistry . Feb2008, Vol. 373 Issue 2, p398-400. 3p. - Publication Year :
- 2008
-
Abstract
- Abstract: A modified polymerase chain reaction (PCR)-based site-directed mutagenesis method used to splice together different regions of a gene by deleting hundreds of nucleotides of undesired sequences is described. This method was inspired by a PCR-based site-directed mutagenesis method developed by Stratagene (La Jolla, CA, USA); the procedure and primer design were modified to enable the method to generate deletions several hundreds of nucleotides in length with an efficiency of 80–100%, and to delete two DNA fragments simultaneously in a single PCR. This method should be useful for deletion of large DNA fragments from a gene. [Copyright &y& Elsevier]
- Subjects :
- *MUTAGENESIS
*POLYMERASE chain reaction
*GENETIC engineering
*NUCLEOTIDES
Subjects
Details
- Language :
- English
- ISSN :
- 00032697
- Volume :
- 373
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Analytical Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 28136240
- Full Text :
- https://doi.org/10.1016/j.ab.2007.10.021