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Extracellular NAD is a regulator for FcγR-mediated phagocytosis in murine macrophages
- Source :
-
Biochemical & Biophysical Research Communications . Feb2008, Vol. 367 Issue 1, p156-161. 6p. - Publication Year :
- 2008
-
Abstract
- Abstract: NAD is available in the extracellular environment and elicits immune modulation such as T cell apoptosis by being used as the substrate of cell surface ADP-ribosyl transferase. However, it is unclear whether extracellular NAD affects function of macrophages expressing cell surface ADP-ribosyl transferase. Here we show that extracellular NAD enhances Fcγ receptor (FcγR)-mediated phagocytosis in J774A.1 macrophages via the conversion into cyclic ADP-ribose (cADPR), a potent calcium mobilizer, by CD38, an ADP-ribosyl cyclase. Extracellular NAD increased the phagocytosis of IgG-coated sheep red blood cells (IgG-SRBC) in J774A.1 macrophages, which was completely abolished by pretreatment of 8-bromo-cADPR, an antagonist of cADPR, or CD38 knockdown. Extracellular NAD increased basal intracellular Ca2+ concentration, which also was abolished by pretreatment of 8-bromo-cADPR or CD38 knockdown. Moreover, the chelation of intracellular calcium abolished NAD-induced enhancement of phagocytosis of IgG-SRBC. Our results suggest that extracellular NAD act as a regulator for FcγR-mediated phagocytosis in macrophages. [Copyright &y& Elsevier]
- Subjects :
- *PHAGOCYTOSIS
*IMMUNOGLOBULIN G
*MACROPHAGES
*APOPTOSIS
Subjects
Details
- Language :
- English
- ISSN :
- 0006291X
- Volume :
- 367
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Biochemical & Biophysical Research Communications
- Publication Type :
- Academic Journal
- Accession number :
- 28552907
- Full Text :
- https://doi.org/10.1016/j.bbrc.2007.12.131