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Tandem MS Can Distinguish Hyaluronic Acid from N-Acetylheparosan
- Source :
-
Journal of the American Society for Mass Spectrometry . Jan2008, Vol. 19 Issue 1, p82-90. 9p. - Publication Year :
- 2008
-
Abstract
- Isobaric oligosaccharides enzymatically prepared from hyaluronic acid (HA) and N-acetylheparosan (NAH), were distinguished using tandem mass spectrometry. The only difference between the two series of oligosaccharides was the linkage pattern (in HA 1→3 and in NAH 1→4) between glucuronic acid and N-acetylglucosamine residues. Tandem mass spectrometry afforded spectra in which glycosidic cleavage fragment ions were observed for both HA and NAH oligosaccharides. Cross-ring cleavage ions 0,2An and 0,2An-h (n is even number) were observed only in GlcNAc residues of NAH oligosaccharides. One exception was an 0,2A2 ion fragment observed for the disaccharide from HA. These cross-ring cleavage fragment ions are useful to definitively distinguish HA and NAH oligosaccharides. [Copyright &y& Elsevier]
- Subjects :
- *OLIGOSACCHARIDES
*SPECTRUM analysis
*MASS spectrometry
*IONS
Subjects
Details
- Language :
- English
- ISSN :
- 10440305
- Volume :
- 19
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Journal of the American Society for Mass Spectrometry
- Publication Type :
- Academic Journal
- Accession number :
- 28611972
- Full Text :
- https://doi.org/10.1016/j.jasms.2007.10.012