Evaluación de la actividad antibacteriana de preservos industriales.

The antibacterial in vitro activity of common used industrial preservatives in Cuba were evaluated. The most effective ones in reaction with the bacteria group were selected. The preservatives were: sodium benzoate, potassium sorbate, Kathon CG, p-chloro-m-cresol and the combination of benzalkonium chloride with EDTA and methylparaben with propylparaben. Nine bacterial collection cultures were used: Bacillus subtilis ATCC 6633, Micrococcus luteus ATCC 9341, Staphylococcus aureus ATCC 6538, Citrobacter freundii ATCC 10625, Escherichia coli ATCC 25922, Escherichia coli ATCC 35150, Klebsiella pneumoniae ATCC13883, Proteus mirabilis ATCC 7002 and Pseudomonas aeruginosa ATCC 27853. The minimal inhibitory concentration values of each product in front of the sensitive strains were determined and the minimal bactericide concentration and the minimal effective concentration values of each product were determined. Sodium benzoate and potassium sorbate did not inhibit the growth of the bacterial species tested. It was demonstrated that the preservatives Kathon CG, p-chloro-m-cresol, benzalkonium chloride with EDTA, methylparaben with propylparaben showed a bacteriostatic effect against all the species tested. Bactericide activity was detected for the combination of methylparaben with propylparaben, against all the species used except for Pseudomonas aeruginosa. The products that showed a higher antibacterial effect were Kathon CG, p-chloro-m-cresol and the combination of benzalkonium chloride with EDTA, as they showed bacteriostatic and bactericide action against all of the bacterial species tested; the minimal effective concentrations values were 0.6; 1.0 and 0.15; 1.0 mg/mL respectively. [ABSTRACT FROM AUTHOR]