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Molecular determinants of Rem2 regulation of N-type calcium channels
- Source :
-
Biochemical & Biophysical Research Communications . Apr2008, Vol. 368 Issue 3, p827-831. 5p. - Publication Year :
- 2008
-
Abstract
- Abstract: Rem2 belongs to the RGK family of small GTPases whose members are known to interact with the voltage gated calcium channel β subunit, and to inhibit or abolish calcium currents. To identify the underlying functional domains of Rem2, we created several N- or C-terminally truncated Rem2 proteins and examined their abilities to interact with the Cav β subunit and to regulate the activities of Cav2.2 N-type calcium channels. Confocal imaging of Rem2 in tsA-201 cells revealed that it contains a membrane-targeting signal in its C-terminus, consistent with previous studies. Co-precipitation assays showed that Cav β3 interaction depends on Rem2 residues 1–123. Only Rem2 proteins that targeted the cell membrane as well as bound the β subunit were able to reduce whole cell calcium currents. [Copyright &y& Elsevier]
- Subjects :
- *CALCIUM channels
*CELL membranes
*CONFOCAL microscopy
*PROTEINS
Subjects
Details
- Language :
- English
- ISSN :
- 0006291X
- Volume :
- 368
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Biochemical & Biophysical Research Communications
- Publication Type :
- Academic Journal
- Accession number :
- 31147496
- Full Text :
- https://doi.org/10.1016/j.bbrc.2008.02.020