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Mouse ES cell-derived cardiac precursor cells are multipotent and facilitate identification of novel cardiac genes.
- Source :
-
Journal of Clinical Investigation . Mar2008, Vol. 118 Issue 3, p894-903. 10p. 3 Color Photographs, 1 Black and White Photograph, 3 Graphs. - Publication Year :
- 2008
-
Abstract
- Although the differentiation of ES cells to cardiomyocytes has been firmly established, the extent to which corresponding cardiac precursor cells can contribute to other cardiac populations remains unclear. To determine the molecular and cellular characteristics of cardiac-fated populations derived from mouse ES (mES) cells, we isolated cardiac progenitor cells (CPCs) from differentiating mES cell cultures by using a reporter cell line that expresses GFP under the control of a cardiac-specific enhancer element of Nkx2-5, a transcription factor expressed early in cardiac development. This ES cell-derived CPC population initially expressed genetic markers of both stem cells and mesoderm, while differentiated CPCs displayed markers of 3 distinct cell lineages (cardiomyocytes, vascular smooth muscle cells, and endothelial cells)--Flk1 (also known as Kdr), c-Kit, and Nkx2-5, but not Brachyury--and subsequently expressed Isl1. Clonally derived CPCs also demonstrated this multipotent phenotype. By transcription profiling of CPCs, we found that mES cell-derived CPCs displayed a transcriptional signature that paralleled in vivo cardiac development. Additionally, these studies suggested the involvement of genes that we believe were previously unknown to play a role in cardiac development. Taken together, our data demonstrate that ES cell-derived CPCs comprise a multipotent precursor population capable of populating multiple cardiac lineages and suggest that ES cell differentiation is a valid model for studying development of multiple cardiac-fated tissues. [ABSTRACT FROM AUTHOR]
- Subjects :
- *HEART cells
*GENES
*STEM cells
*MESODERM
*CELL lines
*HEART metabolism
*ANIMAL experimentation
*CELL culture
*CELL differentiation
*CELLS
*COMPARATIVE studies
*IN situ hybridization
*RESEARCH methodology
*MEDICAL cooperation
*MICE
*RESEARCH
*EVALUATION research
*OLIGONUCLEOTIDE arrays
*GENE expression profiling
Subjects
Details
- Language :
- English
- ISSN :
- 00219738
- Volume :
- 118
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Journal of Clinical Investigation
- Publication Type :
- Academic Journal
- Accession number :
- 31198092
- Full Text :
- https://doi.org/10.1172/JCI33942