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GADD34 gene restores virulence in viral vector used in experimental stroke study.
- Source :
-
Journal of Cerebral Blood Flow & Metabolism . Apr2008, Vol. 28 Issue 4, p747-751. 5p. 1 Color Photograph, 1 Graph. - Publication Year :
- 2008
-
Abstract
- GADD34 is expressed in the ischaemic brain and reverses protein synthesis shutdown. Consequently, GADD34 could have neuroprotective potential in stroke. BHK medium, a replication-deficient HSV viral vector (HSV1716) with no insert or containing full-length GADD34, the N terminal or a conserved portion of the gene, was injected into mouse brain before stroke. Infarct size was 1.0±0.26, 1.19±0.36, 1.5±0.36, 1.3±0.36, and 1.1±0.28 mm3, respectively. The increase in infarct size with full-length GADD34 was statistically significant (P<0.05). Immunohistochemistry confirmed viral protein expression. Tissue culture studies revealed GADD34 gene restored virulence in HSV1716, suggesting that HSV virulence, rather than increased GADD34, exacerbated ischaemic damage.Journal of Cerebral Blood Flow & Metabolism (2008) 28, 747–751; doi:10.1038/sj.jcbfm.9600565; published online 10 October 2007 [ABSTRACT FROM AUTHOR]
- Subjects :
- *CEREBROVASCULAR disease
*ISCHEMIA
*TISSUE culture
*CULTURES (Biology)
*HEMODYNAMICS
Subjects
Details
- Language :
- English
- ISSN :
- 0271678X
- Volume :
- 28
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- Journal of Cerebral Blood Flow & Metabolism
- Publication Type :
- Academic Journal
- Accession number :
- 31394906
- Full Text :
- https://doi.org/10.1038/sj.jcbfm.9600565