Back to Search
Start Over
Novel genes involved in canonical Wnt/ β-catenin signaling pathway in early Ciona intestinalis embryos.
- Source :
-
Development, Growth & Differentiation . May2008, Vol. 50 Issue 4, p215-227. 13p. 6 Color Photographs, 3 Charts. - Publication Year :
- 2008
-
Abstract
- We report here characterization of five genes for novel components of the canonical Wnt/ β-catenin signaling pathway. These genes were identified in the ascidian Ciona intestinalis through a loss-of-function screening for genes required for embryogenesis with morpholinos, and four of them have counterparts in vertebrates. The five genes we studied are as follows: Ci-PGAP1, a Ciona orthologue of human PGAP1, which encodes GPI (glycosylphosphatidylinositol) inositol-deacylase, Ci-ZF278, a gene encoding a C2H2 zinc-finger protein, Ci-C10orf11, a Ciona orthologue of human C10orf11 that encodes a protein with leucine-rich repeats, Ci-Spatial/C4orf17, a single counterpart for two human genes Spatial and C4orf17, and Ci-FLJ10634, a Ciona orthologue of human FLJ10634 that encodes a member of the J-protein family. Knockdown of each of the genes mimicked β-catenin knockdown and resulted in suppression of the expression of β-catenin downstream genes ( Ci-FoxD, Ci-Lhx3, Ci-Otx and Ci-Fgf9/16/20) and subsequent endoderm formation. For every gene, defects in knockdown embryos were rescued by overexpression of a constitutively active form, but not wild-type, of Ci- β-catenin. Dosage-sensitive interactions were found between Ci-β-catenin and each of the genes. These results suggest that these five genes act upstream of or parallel to Ci- β-catenin in the Wnt/ β-catenin signaling pathway in early Ciona embryos. [ABSTRACT FROM AUTHOR]
- Subjects :
- *WNT genes
*WNT proteins
*GENES
*EMBRYOS
*EMBRYOLOGY
Subjects
Details
- Language :
- English
- ISSN :
- 00121592
- Volume :
- 50
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- Development, Growth & Differentiation
- Publication Type :
- Academic Journal
- Accession number :
- 31412919
- Full Text :
- https://doi.org/10.1111/j.1440-169X.2008.01012.x