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Identification and Characterization of an Archaeon-Specific Riboflavin Kinase.
- Source :
-
Journal of Bacteriology . Apr2008, Vol. 190 Issue 7, p43-43. 1p. - Publication Year :
- 2008
-
Abstract
- The riboflavin kinase in Methanocaldococcus jannaschii has been identified as the product of the MJ0056 gene. Recombinant expression of the MJ0056 gene in Escherichia coli led to a large increase in the amount of flavin mononucleotide (FMN) in the E. coli cell extract. The unexpected features of the purified recombinant enzyme were its use of CTP as the phosphoryl donor and the absence of a requirement for added metal ion to catalyze the formation of FMN. Identification of this riboflavin kinase fills another gap in the archaeal flavin biosynthetic pathway. Some divalent metals were found to be potent inhibitors of the reaction. The enzyme represents a unique CTP-dependent family of kinases. [ABSTRACT FROM AUTHOR]
- Subjects :
- *VITAMIN B2
*ESCHERICHIA coli
*ESCHERICHIA
*ENTEROBACTERIACEAE
*GENES
*FLAVINS
Subjects
Details
- Language :
- English
- ISSN :
- 00219193
- Volume :
- 190
- Issue :
- 7
- Database :
- Academic Search Index
- Journal :
- Journal of Bacteriology
- Publication Type :
- Academic Journal
- Accession number :
- 31448523
- Full Text :
- https://doi.org/10.1128/JB.01900-07