The Perfect Method, Part 7: The Gradient Shortcut.

The article discusses ways on using the gradient scouting run to speed the isocratic method for phase separation. The first thing to do is to run the scouting run and examine if it is suitable for the sample. Then, determine the isocratic conditions using the Excel spreadsheet calculator. After the scouting gradient has been run and the isocratic conditions have been determined to be appropriate, one can make the first isocratic run.