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Ultrasonic approach to enhance signal-to-noise in three dimensional microarray
- Source :
-
Analytical Biochemistry . May2008, Vol. 376 Issue 2, p280-282. 3p. - Publication Year :
- 2008
-
Abstract
- Abstract: Three-dimensional polyacrylamide gel microarray (3-D gel microarray) has a strong adsorption for hybridzation probes with fluorescent label, resulting in high fluorescent background noise. The background noise has hampered the broad application of the microarray in scientific research. A low-frequency ultrasound of 40kHz has been successfully employed to efficiently remove the nonspecifically bound fluorescent probes from the 3-D gel microarray. The method can significantly enhance the signal to noise ratio of the 3-D gel microarray and make it work better in single-nucleotide polymorphism genotyping. [Copyright &y& Elsevier]
- Subjects :
- *ULTRASONIC imaging
*POLYACRYLAMIDE
*POLYMERS
*GENETIC polymorphisms
Subjects
Details
- Language :
- English
- ISSN :
- 00032697
- Volume :
- 376
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Analytical Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 31675286
- Full Text :
- https://doi.org/10.1016/j.ab.2008.01.039