Back to Search Start Over

Structure, organization and expression of common carp (Cyprinus carpio L.) SLP-76 gene

Authors :
Huang, Rong
Sun, Xiao-Feng
Hu, Wei
Wang, Ya-Ping
Guo, Qiong-Lin
Source :
Fish & Shellfish Immunology. May2008, Vol. 24 Issue 5, p530-541. 12p.
Publication Year :
2008

Abstract

Abstract: SLP-76 is an important member of the SLP-76 family of adapters, and it plays a key role in TCR signaling and T cell function. Partial cDNA sequence of SLP-76 of common carp (Cyprinus carpio L.) was isolated from thymus cDNA library by the method of suppression subtractive hybridization (SSH). Subsequently, the full length cDNA of carp SLP-76 was obtained by means of 3′ RACE and 5′ RACE, respectively. The full length cDNA of carp SLP-76 was 2007bp, consisting of a 5′-terminal untranslated region (UTR) of 285bp, a 3′-terminal UTR of 240bp, and an open reading frame of 1482bp. Sequence comparison showed that the deduced amino acid sequence of carp SLP-76 had an overall similarity of 34–73% to that of other species homologues, and it was composed of an NH2-terminal domain, a central proline-rich domain, and a C-terminal SH2 domain. Amino acid sequence analysis indicated the existence of a Gads binding site R-X-X-K, a 10-aa-long sequence which binds to the SH3 domain of LCK in vitro, and three conserved tyrosine-containing sequence in the NH2-terminal domain. Then we used PCR to obtain a genomic DNA which covers the entire coding region of carp SLP-76. In the 9.2k-long genomic sequence, twenty one exons and twenty introns were identified. RT-PCR results showed that carp SLP-76 was expressed predominantly in hematopoietic tissues, and was upregulated in thymus tissue of four-month carp compared to one-year old carp. RT-PCR and virtual northern hybridization results showed that carp SLP-76 was also upregulated in thymus tissue of GH transgenic carp at the age of four-months. These results suggest that the expression level of SLP-76 gene may be related to thymocyte development in teleosts. [Copyright &y& Elsevier]

Details

Language :
English
ISSN :
10504648
Volume :
24
Issue :
5
Database :
Academic Search Index
Journal :
Fish & Shellfish Immunology
Publication Type :
Academic Journal
Accession number :
31757187
Full Text :
https://doi.org/10.1016/j.fsi.2007.10.011