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Identification of Escherichia coli YgaF as an L-2-Hydroxyglutarate Oxidase.
- Source :
-
Journal of Bacteriology . Jun2008, Vol. 190 Issue 11, p3793-3798. 6p. - Publication Year :
- 2008
-
Abstract
- YgaF, a protein of previously unknown function in Escherichia coli, was shown to possess noncovalently bound flavin adenine dinucleotide and to exhibit L-2-hydroxyglutarate oxidase activity. The inability of anaerobic, reduced enzyme to reverse the reaction by reducing the product α-ketoglutaric acid is explained by the very high reduction potential (+19 mV) of the bound cofactor. The likely role of this enzyme in the cell is to recover α-ketoglutarate mistakenly reduced by other enzymes or formed during growth on propionate. On the basis of the identified function, we propose that this gene be renamed lhgO. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00219193
- Volume :
- 190
- Issue :
- 11
- Database :
- Academic Search Index
- Journal :
- Journal of Bacteriology
- Publication Type :
- Academic Journal
- Accession number :
- 32532579
- Full Text :
- https://doi.org/10.1128/JB.01977-07