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Oxytocin alleviates hepatic ischemia–reperfusion injury in rats

Authors :
Düşünceli, Fikret
İşeri, Sevgin Ö.
Ercan, Feriha
Gedik, Nursal
Yeğen, Cumhur
Yeğen, Berrak Ç.
Source :
Peptides. Jul2008, Vol. 29 Issue 7, p1216-1222. 7p.
Publication Year :
2008

Abstract

Abstract: Various mechanisms have been proposed for the pathogenesis of postischemic hepatic injury, including the generation of reactive oxygen metabolites. Oxytocin (OT) possesses antisecretory, antiulcer effects, facilitates wound healing and has anti-inflammatory properties. Hepatic ischemia–reperfusion (I/R)-injury was induced by inflow occlusion to median and left liver lobes (∼70%) for 30min of ischemia followed by 1h reperfusion in female Sprague–Dawley rats under anesthesia. I/R group (n =8) was administered intraperitoneally either OT (500μg/kg) or saline at 24 and 12h before I/R and immediately before reperfusion. Sham-operated group that underwent laparotomy without hepatic ischemia served as the control. Rats were decapitated at the end of reperfusion period. Hepatic samples were obtained for the measurement of myeloperoxidase (MPO) activity, malondialdehyde (MDA), glutathione (GSH) and collagen levels and histopathological analysis. Tumor necrosis factor-alfa (TNF-α) and transaminases (SGOT, SGPT) were assayed in serum samples. I/R injury caused significant increases in hepatic microscopic damage scores, MPO activity, collagen levels, transaminase, serum TNF-α levels. Oxytocin treatment significantly reversed the I/R-induced elevations in serum transaminase and TNF-α levels and in hepatic MPO and collagen levels, and reduced the hepatic damage scores. OT treatment had tendency to abolish I/R-induced increase in MDA levels, while GSH levels were not altered. These results suggest that OT has a protective role in hepatic I/R injury and its protective effect in the liver appears to be dependent on its inhibitory effect on neutrophil infiltration. [Copyright &y& Elsevier]

Details

Language :
English
ISSN :
01969781
Volume :
29
Issue :
7
Database :
Academic Search Index
Journal :
Peptides
Publication Type :
Academic Journal
Accession number :
32553468
Full Text :
https://doi.org/10.1016/j.peptides.2008.02.010