Back to Search
Start Over
Janus-Kinase-3-Dependent Signals Induce Chromatin Remodeling at the Ifng Locus during T Helper 1 Cell Differentiation
- Source :
-
Immunity (10747613) . Jun2008, Vol. 28 Issue 6, p763-773. 11p. - Publication Year :
- 2008
-
Abstract
- Summary: Differentiation of naive CD4+ T cells into T helper type 1 (Th1) effector cells requires both T cell receptor (TCR) signaling and cytokines such as interleukin-12 and interferon γ (IFN-γ). Here, we report that a third cytokine signal, mediated by the Janus family tyrosine kinase 3 (Jak3) and signal transducer and activator of transcription 5 (STAT5) pathway, is also required for Th1 cell differentiation. In the absence of Jak3-dependent signals, naive CD4+ T cells proliferate robustly but produce little IFN-γ after Th1 cell polarization in vitro. This defect is not due to reduced activation of STAT1 or STAT4 or to impaired upregulation of the transcription factor T-bet. Instead, we find that T-bet binding to the Ifng promoter is greatly diminished in the absence of Jak3-dependent signals, correlating with a decrease in Ifng promoter accessibility and histone acetylation. These data indicate that Jak3 regulates epigenetic modification and chromatin remodeling of the Ifng locus during Th1 cell differentiation. [Copyright &y& Elsevier]
- Subjects :
- *T cells
*CELL differentiation
*LYMPHOCYTES
*CYTOKINES
Subjects
Details
- Language :
- English
- ISSN :
- 10747613
- Volume :
- 28
- Issue :
- 6
- Database :
- Academic Search Index
- Journal :
- Immunity (10747613)
- Publication Type :
- Academic Journal
- Accession number :
- 32558283
- Full Text :
- https://doi.org/10.1016/j.immuni.2008.04.016