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Transcutaneous pO2 imaging during tourniquet-induced forearm ischemia using planar optical oxygen sensors.
- Source :
-
Skin Research & Technology . Aug2008, Vol. 14 Issue 3, p304-311. 8p. 1 Diagram, 2 Graphs. - Publication Year :
- 2008
-
Abstract
- Background: Oxygen-dependent quenching of luminescence using transparent planar sensor foils was shown to overcome the limitations of the polarographic electrode technique in an animal model. This method was then transferred to a clinical setting to measure the transcutaneous pO2 (ptcO2). Methods: In six healthy subjects, a cuff on the upper arm was occluded up to 20 mmHg above systolic pressure and released after 8 min. PtcO2 was measured at the lower arm every 30 s before, during, and up to 20 min after cuff occlusion (40 °C applied skin temperature) using luminescence lifetime imaging (LLI) of platinum(II)-octaethyl-porphyrin immobilized in a polystyrene matrix. For validation, the polarographic Clark electrode technique was applied in close proximity, and measurements were conducted simultaneously. Results: PtcO2 measurements before (70.8±19.1 vs. 66.2±7.7 mmHg) and at the end of ischemic (2.7±1.2 vs. 3.6±1.7 mmHg) and reperfusion phases (72.2±3.6 vs. 68.4±8.9 mmHg) did not differ significantly using the Clark electrode vs. LLI. At both the initial ischemic and the reperfusion phases, the Clark electrode measured a faster decrease or increase, respectively, in ptcO2 because of the oxygen consumption occurring in this method. Conclusion: The presented method provides accurate and reproducible ptcO2 values under changing microcirculatory conditions. The lack of oxygen consumption during measurement allows both a more realistic estimation of ptcO2 than compared with the gold standard and permanent use in regions with critical oxygen supply. [ABSTRACT FROM AUTHOR]
- Subjects :
- *LUMINESCENCE
*OXYGEN
*ELECTRODES
*CARDIAC contraction
*PLATINUM
Subjects
Details
- Language :
- English
- ISSN :
- 0909752X
- Volume :
- 14
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Skin Research & Technology
- Publication Type :
- Academic Journal
- Accession number :
- 33208392
- Full Text :
- https://doi.org/10.1111/j.1600-0846.2008.00295.x