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Endocytic pathway followed by dengue virus to infect the mosquito cell line C6/36 HT
- Source :
-
Virology . Aug2008, Vol. 378 Issue 1, p193-199. 7p. - Publication Year :
- 2008
-
Abstract
- Abstract: The endocytic pathway followed by dengue virus to infect the mosquito cells C6/36 HT was analyzed. Using DIL-labeled virions and real-time imaging it was determined that viral entry into C6/36 HT takes approximately 5 to 7 min. Pretreatment of C6/36 HT cells with sucrose and bafilomycin A, but not filipin, inhibited dengue virus infection up to 80%. Furthermore, the overexpression of dominant-negative mutants of Eps15, a molecule required for the formation of clathrin-coated vesicles, reduced dengue infection up to 50%, indicating that dengue virus entry is through clathrin-mediated endocytosis and is pH-dependent. By double-immunofluorescence assays, DIL-labeled particles were colocalized with early endosomes at 5 min and with lysosomes mainly at 30 min post-infection. Finally, disruption of the microtubule and microfilaments by nocodazole and by cytochalasin D reduced viral infection by more than 80%. Taken together these results indicate that dengue virions enter into C6/36 HT cells by clathrin-mediated endocytosis, using the endosomal pathway from early endosomes to acidic lysosomes before viral RNA is released into the cytoplasm. [Copyright &y& Elsevier]
- Subjects :
- *DENGUE viruses
*CELL lines
*MOSQUITOES
*ENDOSOMES
*ENDOCYTOSIS
*LYSOSOMES
Subjects
Details
- Language :
- English
- ISSN :
- 00426822
- Volume :
- 378
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Virology
- Publication Type :
- Academic Journal
- Accession number :
- 33467000
- Full Text :
- https://doi.org/10.1016/j.virol.2008.05.012