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Downregulation of CCR5 on activated CD4 T cells in HIV-infected Indians
- Source :
-
Journal of Clinical Virology . Sep2008, Vol. 43 Issue 1, p25-31. 7p. - Publication Year :
- 2008
-
Abstract
- Abstract: Background: HIV infection in India is unique as it occurs predominantly by CCR5-utilizing isolates that exhibit no co-receptor switch. Objectives: To study HIV-1 co-receptor dynamics on T cells and monocytes following viral infection. Study design: HIV co-receptor expression was evaluated by flow cytometry on various cell subsets in HIV-infected Indians and in vitro in human peripheral blood mononuclear cells infected with CCR5- or CXCR4-utilizing HIV-1. Transfection of the T cell line CEM-CCR5 (which expresses CD4, CCR5 and CXCR4) with HIV-1 Nef or Vpu expression vectors, or treatment with recombinant soluble gp120 from CCR5- and CXCR4-tropic HIV-1, was carried out to determine their effects on co-receptor expression. Results: Indian HIV patients had fewer CD4+CCR5+ T cells and CCR5-expressing activated CD4+ T cells, but higher CXCR4-expressing activated CD4+ T cells compared with controls. Expression of CCR5 was not different on monocytes in HIV patients as compared to controls. The CCR5 downregulation on T cells was HIV infection specific and was governed by the co-receptor-utilization phenotype of the virus. The Nef and soluble gp120 proteins induced CCR5 downregulation, the latter in a co-receptor-utilization phenotype specific manner. Conclusions: The HIV-1 co-receptor dynamics in Indian patients is distinct from western patients and depends upon the virus surface protein. We propose this to be a viral survival strategy. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 13866532
- Volume :
- 43
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Journal of Clinical Virology
- Publication Type :
- Academic Journal
- Accession number :
- 33999713
- Full Text :
- https://doi.org/10.1016/j.jcv.2008.03.026