ESTABILIDAD DE LA CEPA IMI SD: 187 DE Pochonia chlamydosporia var. catenulata (KAMYSCHO EX BARRON Y ONIONS) ZARE Y W. GAMS. PARTE II. INDICADORES BIOQUIMICOS.

A fundamental aspect to develop a Biological Control Agent (BCA) is the genetic stability for the selected isolate for mass rearing and its application. Virulence can be attenuated by serial subcultures. The induction of proteins and different enzymes in liquid medium supplemented with gelatine, chitin or lipid was studied in two IMI SD 187 isolate subcultures, in a time course experiment during 3, 5 and 7 days. In general the results show there are not significant differences in the behaviour of isolates and there is not an attenuation effect in the enzymatic activity. The gelatine supplemented medium was a good inductor for all the protein indicators studied. In the chitin medium there was a special induction for VCP 1 protease. The medium with lipids was a good inductor for total proteins, but in general a bad inductor for the enzymes tested. [ABSTRACT FROM AUTHOR]