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Epsin 1 is a cargo-specific adaptor for the clathrin-mediated endocytosis of the influenza virus.
- Source :
-
Proceedings of the National Academy of Sciences of the United States of America . 8/19/2008, Vol. 105 Issue 33, p11790-11795. 6p. 5 Graphs. - Publication Year :
- 2008
-
Abstract
- During clathrin-mediated endocytosis, adaptor proteins recognize specific internalization signals on cargo receptors, either recruiting cargos into clathrin-coated pits (CCPs) or initiating clathrin-coat assembly around the cargo molecules. Here, we identify epsin 1, a clathrin-, ubiquitin-, and phospholipid-interacting protein, as a cargo-specific adaptor for influenza virus entry through the clathrin-mediated pathway. Using live-cell imaging to monitor the entry of individual virus particles, we observed recruitment of epsin 1 to the binding sites of influenza viruses in synchrony with the assembly of CCPs. Epsin 1 knockdown by siRNA significantly inhibited the clathrin-mediated endocytosis of the influenza virus and caused the majority of the virus particles to enter through a clathrin-independent pathway. The same treatment did not affect the entry of several classical ligands for clathrin-mediated endocytosis, including transferrin, LDL, and EGF. Overexpression of the dominant-negative epsin 1 mutant lacking the ubiquitin-interaction motifs nearly completely blocked the clathrin-mediated entry of the influenza virus without affecting transferrin uptake. These results suggest that epsin 1 functions as a cargo-specific adaptor for the clathrin-mediated entry of the influenza virus. [ABSTRACT FROM AUTHOR]
- Subjects :
- *ENDOCYTOSIS
*INFLUENZA viruses
*UBIQUITIN
*MESSENGER RNA
*PHOSPHOLIPIDS
Subjects
Details
- Language :
- English
- ISSN :
- 00278424
- Volume :
- 105
- Issue :
- 33
- Database :
- Academic Search Index
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Publication Type :
- Academic Journal
- Accession number :
- 34182213
- Full Text :
- https://doi.org/10.1073/pnas.0803711105