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Effect of growth conditions on the molecular weight of poly-3-hydroxybutyrate produced by Azotobacter chroococcum 7B.
- Source :
-
Applied Biochemistry & Microbiology . Sep2008, Vol. 44 Issue 5, p482-486. 5p. 5 Charts. - Publication Year :
- 2008
-
Abstract
- It has been shown that poly-3-hydroxybutyrate ( PHB) of predetermined molecular weight can be obtained by varying the growth conditions of the producer strain, Azotobacter chroococcum 7B: pH, temperature, aeration, presence of sodium acetate as an additional carbon source, or growth on crude complex carbon sources (molasses, vinasse, or starch). High-molecular-weight polymer can be obtained at pH 7.0, optimal for the culture (1485 kDa), temperature 30–37°C (1600–1450 kDa, respectively), and low aeration (2215 kDa). The following factors decrease PHB MW: pH deviation to the acidic (pH 6.0, 476 kDa) or alkaline (pH 8.0, 354 kDa) range or lower temperature (20°C, 897 kDa). Introduction of additional carbon source (sodium acetate) at concentrations in the medium varying from 0 to 5 g/l provides an original method of production of PHB with predetermined MW in a wide range, from 270 to 1515 kDa, with high PHB content in the cell. [ABSTRACT FROM AUTHOR]
- Subjects :
- *AZOTOBACTER
*MOLECULAR weights
*POLY-beta-hydroxybutyrate
*SODIUM acetate
*POLYMERS
Subjects
Details
- Language :
- English
- ISSN :
- 00036838
- Volume :
- 44
- Issue :
- 5
- Database :
- Academic Search Index
- Journal :
- Applied Biochemistry & Microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 34204645
- Full Text :
- https://doi.org/10.1134/S0003683808050050