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Genome-wide search for yeast RNase P substrates reveals role in maturation of intron-encoded box CID small nucleolar RNAs.
- Source :
-
Proceedings of the National Academy of Sciences of the United States of America . 8/26/2008, Vol. 105 Issue 34, p12218-12223. 6p. 5 Diagrams, 2 Charts. - Publication Year :
- 2008
-
Abstract
- Ribonuclease P (RNase P) is an essential endonuclease responsible for the 5′-end maturation of precursor tRNAs. Bacterial RNase P also processes precursor 4.55 RNA, tmRNA, 305 preribosomal RNA, and several reported protein-coding RNAs. Eukaryotic nuclear RNase P is far more complex than in the bacterial form, employing multiple essential protein subunits in addition to the catalytic RNA subunit. RNomic studies have shown that RNase P binds other RNAs in addition to tRNAs, but no non-tRNA substrates have previously been identified. Additional substrates were identified by using a multipronged approach in the budding yeast Saccharomyces cerevisiae. First, RNase P-dependant changes in RNA abundance were examined on whole-genome microarrays by using strains containing temperature sensitive (TS) mutations in two of the essential RNase P subunits, Pop1p and Rpr1r. Second, RNase P was rapidly affinity-purified, and copurified RNAs were identified by using a genome-wide microarray. Third, to identify RNAs that do not change abundance when RNase P is depleted but accumulate as larger precursors, >80 potential small RNA substrates were probed directly by Northern blot analysis with RNA from the RNase P TS mutants. Numerous potential substrates were identified, of which we characterized the box C/D intron-encoded small nucleolar RNAs (snoRNAs). because these both copurify with RNase P and accumulate larger forms in the RNase P temperature-sensitive mutants. it was previously known that two pathways existed for excising these snoRNAs, one using the pre-mRNA splicing path and the other that was independent of splicing. RNase P appears to participate in the splicing-independent path for the box C/D intron-encoded snoRNAs. [ABSTRACT FROM AUTHOR]
- Subjects :
- *RIBONUCLEASES
*GENOMES
*YEAST
*RNA
*ENDONUCLEASES
Subjects
Details
- Language :
- English
- ISSN :
- 00278424
- Volume :
- 105
- Issue :
- 34
- Database :
- Academic Search Index
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Publication Type :
- Academic Journal
- Accession number :
- 34248078
- Full Text :
- https://doi.org/10.1073/pnas.0801906105