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Responses of the Medaka HPG Axis PCR Array and Reproduction to Prochloraz and Ketoconazole.
- Source :
-
Environmental Science & Technology . 9/1/2008, Vol. 42 Issue 17, p6762-6769. 8p. - Publication Year :
- 2008
-
Abstract
- Effects of two model imidazole-type fungicides, prochloraz (PCZ) and ketoconazole (KTC), on the hypothalamic-pituitary-gonadal (HPG) axis of the Japanese medaka (Oryzias latipe) were examined by use of real time PCR (RT-PCR) array. Fourteen-week-old Japanese medaka were exposed for seven days to concentrations of PCZ or KTC from 3.0 to 300 μg/L Exposure to KTC or PCZ caused significant reduction of fecundity of Japanese medaka and down-regulated expression of estrogen receptor (ER)-γ and egg precursors in livers of males and females. However, PCZ was more potent than KTC both in modulating transcription and causing lesser fecundity. Exposure to nominal 30 μg PCZ/L resulted in 50% less fecundity and significant down-regulation of vitellogenin // expression, but KTC did not cause such effects at this concentration. Exposure to PCZ caused a compensatory up-regulation in cytochrome P450 c17αhydroxylase, 17,20-lyase (CYP17) and aromatase (CYP19) expression in the ovary, while KTC did not Furthermore, the ecologically relevant end point, fecundity was log-log related to mRNA level of six genes in livers of females. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 0013936X
- Volume :
- 42
- Issue :
- 17
- Database :
- Academic Search Index
- Journal :
- Environmental Science & Technology
- Publication Type :
- Academic Journal
- Accession number :
- 34251923
- Full Text :
- https://doi.org/10.1021/es800591t