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Efficient transformation and expression of gfp gene in the edible mushroom Pleurotus nebrodensis
Efficient transformation and expression of gfp gene in the edible mushroom Pleurotus nebrodensis
- Source :
-
Progress in Natural Science . Jul2008, Vol. 18 Issue 7, p819-824. 6p. - Publication Year :
- 2008
-
Abstract
- Abstract: An efficient transformation method mediated by PEG-protoplasts was developed for the newly commercial edible mushroom Pleurotus nebrodensis. Two plasmids were used to co-transform protoplasts of P. nebrodensis. One plasmid is pAN7-1 containing a positive selectable marker gene hph conferring hygromycin B resistance. Another plasmid is pBlue-GFP containing a reporter gene gfp conferring green fluorescent protein. PCR and Southern blot analysis showed that hph gene or/and gfp gene were integrated into the genome of P. nebrodensis transformants. The transformation efficiency of the positive selectable marker gene hph was 3 transformants per microgram of plasmid pAN7-1 DNA, which was about 30 times higher than that previously reported in thoroughly studied Pleurotus species such as Pleurotus ostreatus. The transformation efficiency of the reporter gene gfp was 9 transformants per microgram of plasmid pBlu-GFP DNA. The co-transformation efficiency was 23.68%. This is the first report that a “reporter” gene, green fluorescent protein gene can be successfully stably expressed in this Pleurotus species. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 10020071
- Volume :
- 18
- Issue :
- 7
- Database :
- Academic Search Index
- Journal :
- Progress in Natural Science
- Publication Type :
- Academic Journal
- Accession number :
- 34303746
- Full Text :
- https://doi.org/10.1016/j.pnsc.2008.01.015