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A planar interdigitated ring electrode array via dielectrophoresis for uniform patterning of cells

Authors :
Hsiung, Lo-Chang
Yang, Chun-Hui
Chiu, Chi-Li
Chen, Chen-Lin
Wang, Yueh
Lee, Hsinyu
Cheng, Ji-Yen
Ho, Ming-Chih
Wo, Andrew M.
Source :
Biosensors & Bioelectronics. Dec2008, p875-881. 7p.
Publication Year :
2008

Abstract

Abstract: Uniform patterning of cells is highly desirable for most cellular studies involving cell–cell interactions but is often difficult in an in vitro environment. This paper presents the development of a collagen-coated planar interdigitated ring electrode (PIRE) array utilizing positive dielectrophoresis to pattern cells uniformly. Key features of the PIRE design include: (1) maximizing length along the edges where the localized maximum in the electric field exists; (2) making the inner gap slightly smaller than the outer gap in causing the electric field strength near the center of a PIRE being generally stronger than that near the outer edge of the same PIRE. Results of human hepatocellular carcinoma cells, HepG2, adhered on a 6×6 PIRE array show that cells patterned within minutes with good uniformity (48±6 cells per PIRE). Cell viability test revealed healthy patterned cells after 24h that were still confined to the collagen-coated PIREs. Furthermore, quantification of fluorescence intensity of living cells shows an acceptable reproducibility of cell viability among PIREs (mean normalized intensity per PIRE was 1±0.138). The results suggest that the PIRE array would benefit applications that desire uniform cellular patterning, and improve both response and reproducibility of cell-based biosensors. [Copyright &y& Elsevier]

Details

Language :
English
ISSN :
09565663
Database :
Academic Search Index
Journal :
Biosensors & Bioelectronics
Publication Type :
Academic Journal
Accession number :
34648846
Full Text :
https://doi.org/10.1016/j.bios.2008.07.027