Stress response of Nidula niveo-tomentosa to UV-A light.

Exposition to UV-A light stimulated the growth and synthesis of raspberry ketone in submerged cultures of the basidiomycete Nidula niveotomentosa. To investigate the fungus' response to UVA light differentially expressed proteins were identified by means of 2D-electrophoresis. Light induced proteins were de novo sequenced by ESI-MS/MS spectrometry, and the encoding nucleotide sequences were cloned from cDNA or genomic DNA. The spectrum of UV-A light-induced proteins comprised several stress-related proteins including a catalase, heat-shock proteins, glutathione s-transferases and proteasomes. In addition, growth-related enzymes of the citric cycle were found to be up-regulated as a response to irradiation with UV-A. [ABSTRACT FROM AUTHOR]