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Terminal structures of West Nile virus genomic RNA and their interactions with viral NS5 protein
- Source :
-
Virology . Nov2008, Vol. 381 Issue 1, p123-135. 13p. - Publication Year :
- 2008
-
Abstract
- Abstract: Genome cyclization is essential for flavivirus replication. We used RNases to probe the structures formed by the 5′-terminal 190 nucleotides and the 3′-terminal 111 nucleotides of the West Nile virus (WNV) genomic RNA. When analyzed individually, the two RNAs adopt stem-loop structures as predicted by the thermodynamic-folding program. However, when mixed together, the two RNAs form a duplex that is mediated through base-pairings of two sets of RNA elements (5′CS/3′CSI and 5′UAR/3′UAR). Formation of the RNA duplex facilitates a conformational change that leaves the 3′-terminal nucleotides of the genome (position −8 to −16) to be single-stranded. Viral NS5 binds specifically to the 5′-terminal stem-loop (SL1) of the genomic RNA. The 5′SL1 RNA structure is essential for WNV replication. The study has provided further evidence to suggest that flavivirus genome cyclization and NS5/5′SL1 RNA interaction facilitate NS5 binding to the 3′ end of the genome for the initiation of viral minus-strand RNA synthesis. [Copyright &y& Elsevier]
- Subjects :
- *WEST Nile virus
*GENOMES
*FLAVIVIRUSES
*VIRAL replication
*NUCLEOTIDES
*RNA
Subjects
Details
- Language :
- English
- ISSN :
- 00426822
- Volume :
- 381
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Virology
- Publication Type :
- Academic Journal
- Accession number :
- 34954482
- Full Text :
- https://doi.org/10.1016/j.virol.2008.07.040