Back to Search
Start Over
DNA-binding and photocleavage properties of cationic porphyrin–anthraquinone hybrids with different lengths of links
- Source :
-
Bioorganic Chemistry . Dec2008, Vol. 36 Issue 6, p278-287. 10p. - Publication Year :
- 2008
-
Abstract
- Abstract: Four cationic porphyrin–anthraquinone (Por–AQ) hybrids differing in lengths of flexible alkyl linkage, 5-[4-(1-N-anthraquinonon-yl)-l-oxophenyl]-10,15,20-tris(N-methylpyridinium-4-yl)porphyrin triiodide, (l =acetyl, pentanoyl, octanoyl, undecanoyl, designed as [AQATMPyP]I3, [AQPTMPyP]I3, [AQOTMPyP]I3 and [AQUTMPyP]I3, respectively, see Fig. 1), were synthesized and their interactions with DNA were investigated. The results of spectroscopic, denaturation and viscosity measurements suggest that [AQATMPyP]I3 binds to DNA through non-intercalative mode while the other three hybrids with longer links bind via bis-intercalative mode. Ethidium bromide (EB) competition experiment was carried out to determine the binding constants (K b) of these compounds for CT DNA, and [AQPTMPyP]I3 shows the largest K b among these hybrids. The photocleavage mechanism and wavelength-dependent cleaving abilities of these hybrids to pBR322 plasmid DNA were also comparably investigated. [Copyright &y& Elsevier]
- Subjects :
- *DNA
*BIOCHEMISTRY
*DNA-binding proteins
*DNA-ligand interactions
Subjects
Details
- Language :
- English
- ISSN :
- 00452068
- Volume :
- 36
- Issue :
- 6
- Database :
- Academic Search Index
- Journal :
- Bioorganic Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 34954489
- Full Text :
- https://doi.org/10.1016/j.bioorg.2008.08.002