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Magnetic beads-based immunoassay as a sensitive alternative for atrazine analysis
- Source :
-
Talanta . Dec2008, Vol. 77 Issue 2, p839-843. 5p. - Publication Year :
- 2008
-
Abstract
- Abstract: A new immunoassay strategy for sensitive atrazine determination based on magnetic beads is reported. The immuno-method is a competitive solid-phase immunoassay where the anti-atrazine antibody is immobilized on the magnetic beads surface and fixed at the reaction cell bottom using a simple magnet, which generates a magnetic field. Analyte and HRP (horseradish peroxidase) tracer compete for active sites of antibody. After the immunointeractions antibody-analyte and antibody-tracer, atrazine quantification from the sample is performed by injection of the chemiluminescence substrate (luminol, hydrogen peroxide and p-iodophenol). Different antibodies (polyIgG anti-atrazine Ab I and affinity purified polyIgG anti-atrazine AbI) were tested in this configuration. Also, optimum concentration of antibody-covered magnetic beads was set up (8mg/l Ab II). Finally, the performance of magnetic beads-based immunoassay for atrazine determination was evaluated demonstrating that the magnetic beads-based immunoassay is one of the most sensitive method for atrazine determination (LoD=3pg/l, IC50 =37pg/l, DR=10–1000pg/l). [Copyright &y& Elsevier]
- Subjects :
- *IMMUNOASSAY
*ATRAZINE
*MAGNETIC fields
*HORSERADISH
Subjects
Details
- Language :
- English
- ISSN :
- 00399140
- Volume :
- 77
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Talanta
- Publication Type :
- Academic Journal
- Accession number :
- 34979286
- Full Text :
- https://doi.org/10.1016/j.talanta.2008.07.040