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Bright-field analysis of phi29 DNA packaging motor using a magnetomechanical system.
- Source :
-
Applied Physics Letters . 10/13/2008, Vol. 93 Issue 15, p153902. 3p. 2 Color Photographs, 1 Diagram, 1 Graph. - Publication Year :
- 2008
-
Abstract
- We report a simple and robust magnetomechanical system for direct visual observation of the DNA packaging behavior of the bacteriophage phi29 in real time. The system comprises a micron-sized magnetic bead attached to the free end of the viral DNA, a magnet and a bright-field microscope. We show that the phi29 DNA packaging activity can be observed and dynamically analyzed at the single molecular level in bright field with a relatively simple system. With this system we also visually demonstrate the phi29 motor transporting a cargo 10 000 times the viral size. [ABSTRACT FROM AUTHOR]
- Subjects :
- *DNA
*BACTERIOPHAGES
*MAGNETISM
*FLUORESCENCE microscopy
*ADENOSINE triphosphate
Subjects
Details
- Language :
- English
- ISSN :
- 00036951
- Volume :
- 93
- Issue :
- 15
- Database :
- Academic Search Index
- Journal :
- Applied Physics Letters
- Publication Type :
- Academic Journal
- Accession number :
- 34984667
- Full Text :
- https://doi.org/10.1063/1.3000606