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Development of cell-expressed and virion-incorporated CCR5-targeted vaccine
- Source :
-
Biochemical & Biophysical Research Communications . Dec2008, Vol. 377 Issue 2, p617-621. 5p. - Publication Year :
- 2008
-
Abstract
- Abstract: Our previous study demonstrated that the immunization with a cycloimmunogen derived from extracellular loop-2 (ECL-2) of CCR5 (cDDR5) attenuated acute phase of CCR5-tropic simian-human immunodeficiency virus (SHIV)SF162P3 replication in vivo. Although the study showed that the antisera raised against cDDR5 reacted with cell-expressed CCR5, we have not yet demonstrated whether the antisera can react with virion-incorporated CCR5. Here, we show that rhesus cDDR5 (rcDDR5)-specific antibodies react with not only cell-expressed but also virion-incorporated simian CCR5s (siCCR5s), but may predominantly exert their inhibitory effects on simian immunodeficiency virus (SIV) infection by the binding of cell-expressed rather than virion-incorporated CCR5s. These results suggest that the virion-incorporated CCR5 may contribute to the reactivation of the anti-rcDDR5 antibody-producing B-cells by SIV particles after rcDDR5 immunization, although the binding of anti-rcDDR5 antibody to virion-incorporated CCR5 results in a partial inhibitory effect on SIV infection. [Copyright &y& Elsevier]
- Subjects :
- *GENE expression
*IMMUNIZATION
*IMMUNE serums
*IMMUNOTHERAPY
*HIV
*SIMIAN viruses
Subjects
Details
- Language :
- English
- ISSN :
- 0006291X
- Volume :
- 377
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Biochemical & Biophysical Research Communications
- Publication Type :
- Academic Journal
- Accession number :
- 35290848
- Full Text :
- https://doi.org/10.1016/j.bbrc.2008.10.022