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Phenotypic Characterization and Invasive Properties of Synovial Fluid-Derived Adherent Cells in Rheumatoid Arthritis.
- Source :
-
Inflammation . Dec2008, Vol. 31 Issue 6, p365-371. 7p. 1 Color Photograph, 3 Charts, 2 Graphs. - Publication Year :
- 2008
-
Abstract
- The present study aimed at characterizing the phenotype and functions of adherent synovial fluid (SF) cells derived from rheumatoid arthritis (RA), comparing with fibroblast-like synoviocytes (FLS) derived from RA synovial tissue (ST). Adherent SF-derived cells were spindle-shaped from passages 1–6 under light microscopy. The cell surface marker profile in SF-derived cells from passage 1–6 was similar to that of ST-derived FLS. Levels of MMP-1 and MMP-3 were not significantly different between SF-derived cells and ST-derived FLS ( p = 0.20 and p = 0.40, respectively). There was no significant difference in the optical density value between two cell types in the cell invasion assay ( p = 0.10). SF-derived adherent cells have a fibroblast-like phenotype from very early culture passages and have the potential to produce MMPs with the invasive capacity to degrade cartilage, identical to ST-derived FLS. Therefore, these cells could substitute for ST-derived FLS in studying the pathogenesis of RA. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 03603997
- Volume :
- 31
- Issue :
- 6
- Database :
- Academic Search Index
- Journal :
- Inflammation
- Publication Type :
- Academic Journal
- Accession number :
- 35387525
- Full Text :
- https://doi.org/10.1007/s10753-008-9087-x