Back to Search
Start Over
The Role of PI3/Akt Pathway in the Protective Effect of Insulin against Corticosterone Cell Death Induction in Hippocampal Cell Culture.
- Source :
-
Neuroendocrinology . Oct2008, Vol. 88 Issue 4, p293-298. 6p. 4 Graphs. - Publication Year :
- 2008
-
Abstract
- Corticosterone induces neuroanatomical and neurochemical changes in the hippocampus that are associated with cognitive impairments. In the present study corticosterone induced cell death in primary hippocampal neurons cultured in Neurobasal + B27 medium. Insulin prevents neuronal cell death induced in a concentration dependent manner. The neuroprotective effect of insulin was reversed by LY294002, a phosphatidylinositol 3′-kinase (PI3 kinase) inhibitor, whereas the mitogen-activated protein kinase (MAPK) inhibitor PD98059, an upstream blocker of MAPK had no effect. Western blot analyses showed that insulin induced the activation of protein kinase B (Akt). These results suggest that insulin can prevent neuronal cell death induced by corticosterone in hippocampal neurons by modulating the activity of the PI3 kinase/Akt pathway. Copyright © 2008 S. Karger AG, Basel [ABSTRACT FROM AUTHOR]
- Subjects :
- *CORTICOSTERONE
*HIPPOCAMPUS (Brain)
*NEURONS
*INSULIN
*CELL death
Subjects
Details
- Language :
- English
- ISSN :
- 00283835
- Volume :
- 88
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- Neuroendocrinology
- Publication Type :
- Academic Journal
- Accession number :
- 35486852
- Full Text :
- https://doi.org/10.1159/000150441