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Epigallocatechin gallate is a slow-tight binding inhibitor of enoyl-ACP reductase from Plasmodium falciparum
- Source :
-
Biochemical & Biophysical Research Communications . Dec2008, p1238-1242. 5p. - Publication Year :
- 2008
-
Abstract
- Abstract: Epigallocatechin gallate (EGCG) is known to have numerous pharmacological properties. In the present study, we have shown that EGCG inhibits enoyl–acyl carrier protein reductase of Plasmodium falciparum (PfENR) by following a two-step, slow, tight-binding inhibition mechanism. The association/isomerization rate constant (k 5) of the reversible and loose PfENR–EGCG binary complex to a tight [PfENR–EGCG]∗ or EI∗ complex was calculated to be 4.0×10−2 s−1. The low dissociation rate constant (k 6) of the [PfENR–EGCG]∗ complex confirms the tight-binding nature of EGCG. EGCG inhibited PfENR with the overall inhibition constant (K i ∗) of 7.0±0.8nM. Further, we also studied the effect of triclosan on the inhibitory activity of EGCG. Triclosan lowered the k 6 of the EI∗ complex by 100 times, lowering the overall K i ∗ of EGCG to 97.5±12.5 pM. The results support EGCG as a promising candidate for the development of tea catechin based antimalarial drugs. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 0006291X
- Database :
- Academic Search Index
- Journal :
- Biochemical & Biophysical Research Communications
- Publication Type :
- Academic Journal
- Accession number :
- 35512751
- Full Text :
- https://doi.org/10.1016/j.bbrc.2008.10.135