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Activity of Lambda-Exonuclease on Surface-Attached Oligonucleotide Detected by Acoustic Wave Device and Radiochemical Labeling.
- Source :
-
Analytical Letters . 2008, Vol. 41 Issue 15, p2805-2818. 14p. 2 Charts, 3 Graphs. - Publication Year :
- 2008
-
Abstract
- Single-strand 25-mer oligonucleotides have been attached to the surface of a 9-MHz acoustic wave sensor incorporated into a flow-through configuration. This was achieved by an online interaction of the biotinylated probe with chemisorbed neutravidin. Duplex formation with complementary 25-mer was monitored by acoustic wave detection in real time. Regeneration of the probe-modified surface was achieved using online introduction of λ-exonuclease, an enzyme that digests a single strand of double-stranded DNA beginning with the 5' end, cleaving single nucleotides as it progresses. Thus, with the 5' end of the probe strand remaining inaccessible because of the biotin-neutravidin linkage, the target strand was successfully removed by enzymatic digestion. The results of the acoustic wave work are correlated with analogous experiments involving oligonucleotides labeled with 32P. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00032719
- Volume :
- 41
- Issue :
- 15
- Database :
- Academic Search Index
- Journal :
- Analytical Letters
- Publication Type :
- Academic Journal
- Accession number :
- 35523606
- Full Text :
- https://doi.org/10.1080/00032710802418893