Back to Search
Start Over
A Simple Polymerase Chain Reaction-Based Method for the Construction of Recombinase-Mediated Cassette Exchange Donor Vectors.
- Source :
-
Genetics . Nov2008, Vol. 180 Issue 3, p1763-1766. 4p. - Publication Year :
- 2008
-
Abstract
- Here we describe a simple method for generating donor vectors suitable for targeted transgenesis via recombinase-mediated cassette exchange (RMCE) using the ΦC31 integrase. This PCR-based strategy employs small attB "tails" on the primers used to ampIify a sequence of interest, permitting the rapid creation of transgenes for in vivo analysis. [ABSTRACT FROM AUTHOR]
- Subjects :
- *GENERATING functions
*DISEASE vectors
*TRANSGENES
*BIOLOGY
*GENES
Subjects
Details
- Language :
- English
- ISSN :
- 00166731
- Volume :
- 180
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Genetics
- Publication Type :
- Academic Journal
- Accession number :
- 35950201
- Full Text :
- https://doi.org/10.1534/genetics.108.094508