Effect of fluoridated bleaching gels on the remineralization of predemineralized bovine enamel in vitro

Abstract: Objectives: This study evaluated possible differences regarding the remineralization of predemineralized enamel after exposure to fluoridated or non-fluoridated bleaching gels. Methods: 120 enamel specimens were prepared from sixty bovine incisors; before and after demineralization (37°C; pH 4.95; 10d), one-quarter of each specimen''s surface was covered with nail varnish (control sound/demineralized). Subsequently, the specimens were stored for 16h daily in a remineralizing solution (pH 7.0; 14d), while for the remaining time (8h) various bleaching gels were applied: (1) no treatment (control), (2) Opalescence regular (O, Ultradent), (3) Opalescence PF (O-PF), (4) Nite White ACP (NW-ACP, Discus Dental) and (5) Nite White ACPF (NW-ACPF). Following, half of the bleached parts were nail-varnished and stored for another 3-weeks period in a remineralizing solution. Differences in mineral losses (ΔΔZ) and lesion depths (ΔLD) before and after treatment/remineralization period were evaluated from microradiographs. ΔΔZ surface values (mineral loss of the outer 18μm of the lesion) were calculated. Results: After 2 and 5 weeks ΔΔZ/ΔLD values of the bleaching groups did not differ significantly from the controls. Treatment with NW-ACPF for 2 weeks resulted in significantly lower ΔΔZ values compared to NW-ACP (p =0.032) and NW-ACPF in higher values than Opalescence regular (p =0.006). Two weeks treatment with O and O-PF resulted in decreased ΔΔZ surface values compared to control (p <0.0005), whereas with NW-ACPF no significant differences could be observed (p =0.062). Application of NW-ACP induced significantly increased ΔΔZ surface values compared to control (p =0.001). Conclusions: No supporting influence of fluoride-containing bleaching gels on remineralization could be observed. [Copyright &y& Elsevier]