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Simultaneous intracellular delivery of targeting antibodies and functional nanoparticles with engineered protein G system
- Source :
-
Biomaterials . Feb2009, Vol. 30 Issue 6, p1197-1204. 8p. - Publication Year :
- 2009
-
Abstract
- Abstract: Cellular internalization of functional nanoparticles that have optical and magnetic properties is very important in the cellular imaging and manipulation of specifically targeted biomolecules. In this study, a robust method to deliver functional nanoparticles and targeting antibodies into cells was suggested. The engineered protein G system, which contains an affinity tag and a cell penetration peptide in the N- and C-terminals, respectively, can capture surface-modified nanoparticles and antibodies without chemical reaction, and then non-invasively deliver them into the cells. Finally, gold-coated iron oxide nanoparticle/engineered protein G hybrid systems were successfully employed as multifunctional cargo systems for the targeting, imaging, and manipulation of mitochondria. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 01429612
- Volume :
- 30
- Issue :
- 6
- Database :
- Academic Search Index
- Journal :
- Biomaterials
- Publication Type :
- Academic Journal
- Accession number :
- 36021530
- Full Text :
- https://doi.org/10.1016/j.biomaterials.2008.11.007