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Characterization, expression profiles, intracellular distribution and association analysis of porcine PNAS-4 gene with production traits.

Authors :
Delin Mo
Zhengmao Zhu
te Pas, Marinus F. W.
Xinyun Li
Shulin Yang
Heng Wang
Huanling Wang
Kui Li
Source :
BMC Genetics. 2008, Vol. 9, Special section p1-10. 10p. 1 Color Photograph, 2 Diagrams, 3 Charts, 2 Graphs.
Publication Year :
2008

Abstract

Background: In a previous screen to identify differentially expressed genes associated with embryonic development, the porcine PNAS-4 gene had been found. Considering differentially expressed genes in early stages of muscle development are potential candidate genes to improve meat quality and production efficiency, we determined how porcine PNAS-4 gene regulates meat production. Therefore, this gene has been sequenced, expression analyzed and associated with meat production traits. Results: We cloned the full-length cDNA of porcine PNAS-4 gene encoding a protein of 194 amino acids which was expressed in the Golgi complex. This gene was mapped to chromosome 10, q11-16, in a region of conserved synteny with human chromosome 1 where the human homologous gene was localized. Real-time PCR revealed that PNAS-4 mRNA was widely expressed with highest expression levels in skeletal muscle followed by lymph, liver and other tissues, and showed a down-regulated expression pattern during prenatal development while a up-regulated expression pattern after weaning. Association analysis revealed that allele C of SNP A1813C was prevalent in Chinese indigenous breeds whereas A was dominant allele in Landrace and Large White, and the pigs with homozygous CC had a higher fat content than those of the pigs with other genotypes (P < 0.05). Conclusion: Porcine PNAS-4 protein tagged with green fluorescent protein accumulated in the Golgi complex, and its mRNA showed a widespread expression across many tissues and organs in pigs. It may be an important factor affecting the meat production efficiency, because its down-regulated expression pattern during early embryogenesis suggests involvement in increase of muscle fiber number. In addition, the SNP A1813C associated with fat traits might be a genetic marker for molecular-assisted selection in animal breeding. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
14712156
Volume :
9
Database :
Academic Search Index
Journal :
BMC Genetics
Publication Type :
Academic Journal
Accession number :
36086237
Full Text :
https://doi.org/10.1186/1471-2156-9-40