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High through-put aflatoxin determination in plant material by automated solid-phase extraction on-line coupled to laser-induced fluorescence screening and determination by liquid chromatography–triple quadrupole mass spectrometry
- Source :
-
Journal of Chromatography A . Feb2009, Vol. 1216 Issue 7, p1115-1125. 11p. - Publication Year :
- 2009
-
Abstract
- Abstract: A screening test based on laser-induced fluorescence (LIF) and a method for individual identification – quantitation of aflatoxins (AFs) in olive leaves and drupes, based on chromatographic separation and triple-quad mass-spectrometry detection with electrospray ionization in positive mode, is here reported. The sensitivity and selectivity of both methods are enhanced by a preconcentration–cleanup step developed by a Prospekt station. The analysis frequency is at least 3.5 samples/h. The screening test makes able to detect the target analytes at concentrations of 0.7μg/kg without “false negatives”. The LC–MS/MS method provides limits of detection (LOD) and quantification (LOQ) ranging between 0.01–0.03 and 0.03–0.11μg/kg, respectively. The linear dynamic range is between LOQ–50μg/kg. The between-day precision, expressed as relative standard deviation, ranges between 0.97–2.86% and the within laboratory reproducibility, also expressed as RSD, between 1.63% and 4.84%. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 00219673
- Volume :
- 1216
- Issue :
- 7
- Database :
- Academic Search Index
- Journal :
- Journal of Chromatography A
- Publication Type :
- Academic Journal
- Accession number :
- 36192524
- Full Text :
- https://doi.org/10.1016/j.chroma.2008.12.049