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In Vivo Tumor Targeting by the B-Subunit of Shiga Toxin.
- Source :
-
Molecular Imaging . Nov/Dec2008, Vol. 7 Issue 6, p239-247. 9p. 5 Diagrams, 2 Graphs. - Publication Year :
- 2008
-
Abstract
- Delivery of drugs to the appropriate target cells would improve efficacy and reduce potential side effects. The nontoxic B-subunit of the intestinal pathogen-produced Shiga toxin (STxB) binds specifically to the glycosphingolipid Gb³, overexpressed in membranes of certain tumor cells, and enters these cells through the retrograde pathway. Therefore, STxB binding to Gb³ receptors may be useful for cell-specific vectorization or imaging purposes. Here we labeled STxB with a fluorophore to evaluate its potential as an in vivo cell-specific targeting reagent in two different models of human colorectal carcinoma. Fluorescent STxB was administered systemically to xenografted nude mice, and its biodistribution was studied by optical imaging. The use of fluorescent STxB allowed the combination of the macroscopic observations with analyses at the cellular level using confocal microscopy. After administration, the fluorescent STxB was slowly eliminated by renal excretion. However, it accumulated in the tumor area. Furthermore, STxB was demonstrated to enter the Gb³-expressing tumoral cells, as well as the epithelial cells of the neovascularization and the monocytes and macrophages surrounding the xenografts. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 15353508
- Volume :
- 7
- Issue :
- 6
- Database :
- Academic Search Index
- Journal :
- Molecular Imaging
- Publication Type :
- Academic Journal
- Accession number :
- 36411818
- Full Text :
- https://doi.org/10.2310/7290.2008.00022