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The prostate transglutaminase (TGase-4, TGaseP) regulates the interaction of prostate cancer and vascular endothelial cells, a potential role for the ROCK pathway
- Source :
-
Microvascular Research . Mar2009, Vol. 77 Issue 2, p150-157. 8p. - Publication Year :
- 2009
-
Abstract
- Abstract: Prostate transglutaminase (TGase-4 or TGaseP) is an enzyme that is uniquely expressed in prostate tissues. The function of the TGase, implicated in the cell-matrix, is yet to be fully established. In the present study, we investigated the role of TGase-4 in tumor-endothelial cell interactions, by creating a panel of prostate cancer cell lines that have different expression profiles of human TGase-4. Here, we report that prostate cancer cells PC-3, when over-expressing TGase-4 (PC-3TGase4exp) increased their ability to adhere to quiescent and activated (by hepatocyte growth factor) endothelial cells. In contrast, the prostate cancer cell CAHPV-10, which expressed high levels of TGase-4, reduced the adhesiveness to the endothelial cells after TGase-4 expression was knocked down. By using frequency based electric cell impedance sensing, we found that TGase-4 mediated adhesion resulted in a change in impedance at low frequency (400 Hz), indicating a paracellular pathway disruption. The study further showed that expression of TGase-4 rendered the cells to exert regulation of endothelial interaction by bypassing the ROCK pathway. It is therefore concluded, that TGase-4 plays a pivotal role in the interaction between endothelial cells and prostate cancer cells, an action which is independent of the ROCK pathway. [Copyright &y& Elsevier]
- Subjects :
- *PROSTATE cancer
*TRANSGLUTAMINASES
*ENDOTHELIUM physiology
*GROWTH factors
Subjects
Details
- Language :
- English
- ISSN :
- 00262862
- Volume :
- 77
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Microvascular Research
- Publication Type :
- Academic Journal
- Accession number :
- 36682748
- Full Text :
- https://doi.org/10.1016/j.mvr.2008.09.010