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A liquid crystal-based sensor for real-time and label-free identification of phospholipase-like toxins and their inhibitors
- Source :
-
Biosensors & Bioelectronics . Mar2009, Vol. 24 Issue 7, p2289-2293. 5p. - Publication Year :
- 2009
-
Abstract
- Abstract: We report a liquid crystal (LC)-based sensor for real-time and label-free identification of phospholipase-like toxins. Beta-bungarotoxin exhibits Ca2+-dependent phospholipase A2 activity whereas alpha-bungarotoxin and myotoxin II do not exhibit any phospholipase activity. The sensor can selectively identify beta-bungarotoxin, when it hydrolyzes a phospholipid monolayer self-assembled at aqueous–LC interface, through orientational responses of LCs. As a result, optical signals that reflect the spatial and temporal distribution of phospholipids during the hydrolysis can therefore be generated in a real-time manner. The sensor is very sensitive and requires less than 5pg of beta-bungarotoxin for the detection. When phospholipase A2 inhibitors are introduced together with beta-bungarotoxin, no orientational response of LCs can be observed. In addition, the regeneration of the sensor can be done without affecting the sensing performance. This work demonstrates a simple and cost-effective LC-based sensor for identifying phospholipase-like toxins and for screening compound libraries to find potential toxin inhibitors. [Copyright &y& Elsevier]
- Subjects :
- *BIOSENSORS
*LIQUID crystals
*PHOSPHOLIPASES
*TOXINS
*CHEMICAL inhibitors
*HYDROLYSIS
Subjects
Details
- Language :
- English
- ISSN :
- 09565663
- Volume :
- 24
- Issue :
- 7
- Database :
- Academic Search Index
- Journal :
- Biosensors & Bioelectronics
- Publication Type :
- Academic Journal
- Accession number :
- 36768213
- Full Text :
- https://doi.org/10.1016/j.bios.2008.11.021