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Mycophenolic acid production in solid-state fermentation using a packed-bed bioreactor
- Source :
-
Biochemical Engineering Journal . May2009, Vol. 44 Issue 2/3, p106-110. 5p. - Publication Year :
- 2009
-
Abstract
- Abstract: Mycophenolic acid (MPA) was produced from Penicillium brevicompactum by solid-state fermentation (SSF) using pearl barley, and submerged fermentation (SmF) using mannitol. It was found that SSF was superior to SmF in terms of MPA concentration (1219mg/L vs. 60mg/L after 144h fermentation), and the product yields were 6.1mg/g pearl barley for SSF and 1.2mg/g mannitol for SmF. The volumetric productivities were 8.5 and 0.42mg/Lh for SSF and SmF, respectively. The optimum solid substrate of SSF for MPA production was pearl barley, producing 5470mg/kg compared with wheat bran (1601mg/kg), oat (3717mg/kg) and rice (2597mg/kg). The optimum moisture content, incubation time and inoculum concentrations were 70%, 144h and 6%, respectively. Neither the addition of mannitol or (NH4)2HPO4 nor adjustment of media pH within the range of 3–7 significantly enhanced MPA production. MPA production by SSF using a packed-bed bioreactor was performed and an increased maximum production of MPA 6.9mg/g was achieved at 168h incubation time. The higher volumetric productivity and concentrations makes SSF an attractive alternative to SmF for MPA production. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 1369703X
- Volume :
- 44
- Issue :
- 2/3
- Database :
- Academic Search Index
- Journal :
- Biochemical Engineering Journal
- Publication Type :
- Academic Journal
- Accession number :
- 36904976
- Full Text :
- https://doi.org/10.1016/j.bej.2008.11.005