Rapid Identification and Determination of the Rodenticide Valone in Serum by High-Performance Liquid Chromatography—Tandem Mass Spectrometry.

The article focuses on the development of a selective and sensitive method to identify and quantify rodenticide valone in serum using high-performance liquid chromatography (HPLC)-tandem mass spectrometry (TMS). Analysis of six extracts of blank human serum samples and an extract spiked only with the internal standard (IS) were used to examine the specificity of the method. Electrospray ionization ion trap mass spectrometry in the negative multiple reaction monitoring mode was used to detect valone in serum. Results show that the identification and quantification of valone in both clinical and forensic specimens was enabled in the proposed method since it combines a rapid solid-phase extraction (SPE) procedure with an extremely fast chromatographic analysis.