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INF1 Elicitin Activates Jasmonic Acid- and Ethylene-mediated Signalling Pathways and Induces Resistance to Bacterial Wilt Disease in Tomato.

Authors :
KAWAMURA, YOKO
HASE, SHU
TAKENAKA, SHIGEHITO
KANAYAMA, YOSHINORI
YOSHIOKA, HIROFUMI
KAMOUN, SOPHIEN
TAKAHASHI, HIDEKI
Source :
Journal of Phytopathology. May2009, Vol. 157 Issue 5, p287-297. 11p. 1 Color Photograph, 4 Black and White Photographs, 2 Charts, 2 Graphs.
Publication Year :
2009

Abstract

Phytophthora infestans, the cause of potato and tomato late blight disease, produces INF1 elicitin, a 10 kDa extracellular protein. INF1 induces a hypersensitive response (HR) and systemic acquired resistance in species of the Nicotiana genus and a few other genera. We analysed the response of tomato to INF1 and INF1 S3, which has a Cys to Ser substitution at position 3 of the processed protein and therefore lacks HR induction activity in tobacco. No HR cell death was induced in either INF1- or INF1 S3-treated tomato leaves. The expression of salicylic acid (SA)-responsive PR-1a( P6) and PR-2a genes was not induced by treatment with either INF1 or INF1 S3. However, the expression of jasmonic acid (JA)-responsive PR-6 encoding proteinase inhibitor II, LeATL6 encoding ubiquitin ligase E3, and LOX-E encoding lipoxygenase, was up-regulated in tomato leaves treated with INF1 but not in those treated with INF1 S3. Their induction was completely compromised in INF1-treated jai1-1 mutant tomato, in which the JA signalling pathway is impaired. The accumulation of ethylene (ET) and the expression of ET-responsive genes were also induced in tomato by INF1 but not INF1 S3 treatment. The activation of JA and ET-mediated signals but not the SA-mediated signalling in INF1-treated tomato was also demonstrated by global gene expression analysis. INF1-treated tomatoes, but not those treated with INF1 S3, exhibited resistance to bacterial wilt disease caused by Ralstonia solanacearum. Thus, INF1 seems to induce resistance to bacterial wilt disease in tomato and activate JA- and ET-mediated signalling pathways without development of HR cell death. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
09311785
Volume :
157
Issue :
5
Database :
Academic Search Index
Journal :
Journal of Phytopathology
Publication Type :
Academic Journal
Accession number :
37307684
Full Text :
https://doi.org/10.1111/j.1439-0434.2008.01489.x