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Sensitive, specific polymorphism discovery in bacteria using massively parallel sequencing.
- Source :
-
Nature Methods . Jan2009, Vol. 6 Issue 1, p67-69. 3p. 2 Charts. - Publication Year :
- 2009
-
Abstract
- Our variant ascertainment algorithm, VAAL, uses massively parallel DNA sequence data to identify differences between bacterial genomes with high sensitivity and specificity. VAAL detected ∼98% of differences (including large insertion-deletions) between pairs of strains from three species while calling no false positives. VAAL also pinpointed a single mutation between Vibrio cholerae genomes, identifying an antibiotic's site of action by identifying sequence differences between drug-sensitive strains and drug-resistant derivatives. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 15487091
- Volume :
- 6
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Nature Methods
- Publication Type :
- Academic Journal
- Accession number :
- 37381400
- Full Text :
- https://doi.org/10.1038/nmeth.1286