Back to Search
Start Over
Highly efficient synthesis of ethyl hexanoate catalyzed by CALB-displaying Saccharomyces cerevisiae whole-cells in non-aqueous phase
- Source :
-
Journal of Molecular Catalysis B: Enzymatic . Jul2009, Vol. 59 Issue 1-3, p168-172. 5p. - Publication Year :
- 2009
-
Abstract
- Abstract: A recombinant Saccharomyces cerevisiae displaying Candida antarctica lipase B (CALB) on the cell surface was constructed and used as a whole-cell biocatalyst to catalyze the esterification of hexanoic acid and ethanol for the preparation of ethyl hexanoate, a fragrance compound of liquor. Various reaction parameters affecting the esterification catalyzed by CALB-displaying S. cerevisiae whole-cells were investigated. The optimal reaction conditions were reaction temperature 40°C, hexanoic acid concentration 0.2M, the ratio of hexanoic acid to ethanol 1:1.25 and the amount of cells 60g/l (17U/g-dry cell). Molecular sieves (3Å) were added to the reaction medium as the water absorbent. The yield reached 98.2% after reaction for 12h under the optimal conditions. The CALB-displaying S. cerevisiae whole-cell biocatalyst exhibited quite a good operational stability in the esterification, and more than 95% of its original activity was retained after 10 batches reaction. Thus, CALB-displaying S. cerevisiae whole-cell biocatalyst is promising for esters synthesis in non-aqueous phase. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 13811177
- Volume :
- 59
- Issue :
- 1-3
- Database :
- Academic Search Index
- Journal :
- Journal of Molecular Catalysis B: Enzymatic
- Publication Type :
- Academic Journal
- Accession number :
- 37817472
- Full Text :
- https://doi.org/10.1016/j.molcatb.2009.02.007