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Corneal crosslinking: Riboflavin concentration in corneal stroma exposed with and without epithelium
- Source :
-
Journal of Cataract & Refractive Surgery . May2009, Vol. 35 Issue 5, p893-899. 7p. - Publication Year :
- 2009
-
Abstract
- Purpose: To evaluate intrastromal concentrations of riboflavin with and without epithelium to ensure the efficacy and safety of corneal crosslinking (CXL) by the standard and transepithelial procedures. Setting: Department of Ophthalmology and Department of Pharmacology G. Segre, Siena University, Siena, Italy. Methods: This study comprised keratoconic patients enrolled for penetrating keratoplasty (PKP) and warm-stored sclerocorneal rings unsuitable for transplantation. Half the PKP specimens were debrided, and half were left with the epithelium in situ. One of the latter and 1 debrided sample were not exposed to riboflavin (controls). Samples in both groups were soaked with 0.1% riboflavin–dextran 20% solution instilled every 2 minutes for 5, 15, and 30 minutes. Riboflavin concentrations were determined by high-performance liquid chromatography (HPLC). Results: The study evaluated 14 PKP specimens and 16 sclerocorneal rings. Control samples did not show a riboflavin emission peak. In exposed samples with epithelium, the mean riboflavin concentration was 91.88 ng/g after 5 minutes of exposure, 95.60 ng/g after 15 minutes, and 94.92 ng/g after 30 minutes. In the debrided samples, the mean riboflavin concentration was 14.42 μg/g, 20.92 μg/g, and 24.06 μg/g, respectively. No differences were seen between the in vivo samples and the ex vivo samples. Conclusions: The HPLC quantitative study showed that stromal concentrations of riboflavin increased with exposure time only if the epithelium was removed. A theoretically safe and effective riboflavin concentration of 15 μg/g was obtained for ultraviolet A-induced CXL only after the epithelium was removed and after at least 10 minutes of riboflavin application every 2 minutes. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 08863350
- Volume :
- 35
- Issue :
- 5
- Database :
- Academic Search Index
- Journal :
- Journal of Cataract & Refractive Surgery
- Publication Type :
- Academic Journal
- Accession number :
- 38320091
- Full Text :
- https://doi.org/10.1016/j.jcrs.2009.01.009