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Quantitative telomerase activity in circulating human leukocytes: utility of real-time telomeric repeats amplification protocol (RQ-TRAP) in a clinical/epidemiological setting.
- Source :
-
Clinical Chemistry & Laboratory Medicine . Jul2009, Vol. 47 Issue 7, p870-873. 4p. 1 Chart, 2 Graphs. - Publication Year :
- 2009
-
Abstract
- Background: There is accumulating evidence from the epidemiological field of telomere biology that telomere length plays an important role in the pathophysiology of cardiovascular disease. The RNA-dependent DNA polymerase, telomerase, is essential in regulating telomere length by acting as a reverse transcriptase. However, the relationship between telomerase activity and telomere length in cardiovascular disease is unclear. This is due, in part, to the paucity of information on the utility of a quantitative and routine assay for the determination of telomerase activity in circulating blood leukocytes. Methods: We used a validated, high-sensitive real-time quantitative telomeric repeat amplification protocol (RQ-TRAP) to determine telomerase activity in circulating blood leukocytes. Results: The present investigation demonstrated direct and reliable detection of telomerase activity of circulating blood leukocytes. Conclusion: The present investigation suggests the feasibility of using RQ-TRAP assay in routine screening of telomerase activity in blood specimens typically collected in a clinical/epidemiological setting. Clin Chem Lab Med 2009;47:870–3. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 14346621
- Volume :
- 47
- Issue :
- 7
- Database :
- Academic Search Index
- Journal :
- Clinical Chemistry & Laboratory Medicine
- Publication Type :
- Academic Journal
- Accession number :
- 42967219
- Full Text :
- https://doi.org/10.1515/CCLM.2009.188